Temporary fixed prostheses are an important adjunct because they protect prepared teeth and prevent exposure of dental tissues. The most commonly used temporary restorative materials in prosthodontics are PMMA, MMA, and the more modern bis-acryl composites. The main techniques for temporary restorations use custom-made and preformed materials. Both are adapted to dental tissues by means of direct clinical, indirect laboratory, and/or combined direct/indirect techniques.

Fig. 1. A. qRT-PCR for IL-6 gene (3 min vs. 30 min). B. qRT-PCR for gp80 and gp130 genes (3 min vs. 30 min).

我直接的临床技术,丙烯酸树脂n close contact with soft tissue during the polymerization phase (3-5 min), and complete polymerization requires 24-72h. In this report, we studied the effects of three commercial polyacrylic resins (Jet Kit, Unifast, and Duralay) on transcriptional control of gene expressions of human IL-6 and its receptors gp80 and gp130 in HGFs. As compared with HGFs processed with resins polymerized for 3 min, gene expression was increased in HGFs treated when these materials were polymerized for 30 min. Moreover, Jet Kit and Unifast had the greatest ability to upregulate IL-6, gp80, and gp130 gene expression. In contrast, Duralay had no effect on transcriptional control of IL-6 or its receptors. Polyacrylic resins induce an inflammatory mechanism in HGFs, and this process is mediated by IL-6 through the gp80 and gp10 receptors. We demonstrated that upregulation of the IL-6, gp80, and gp130 genes in HGFs differs, perhaps because of variation in the characteristics of the acid resins, namely, the amount of the materials released, the composition of the materials, and polymerization time. All these factors have crucial roles in the control of DNA transcription.

In conclusion, the present findings suggest we cannot exclude the involvement of these signals in the activation of inflammatory processes in the human gingival. The present data should be regarded as preliminary and will require further study and experiments, which are currently underway. Specifically, our target is to identify the role of polyacrylic acid resins in the control of acute-phase response.

Spagnuolo Gianrico,Rengo Sandro,Michele Simeone,Francesco RiccitielloandAlfredo Procino
Medical School “Federico II” of Naples, Italy
Department of Neurosciences, Reproductive and Odontostomatological Sciences, Italy

Publication

Polyacrylic resins regulate transcriptional control of interleukin-6, gp80, and gp130 genes in human gingival fibroblasts.
Borelli B, Zarone F, Rivieccio V, Riccitiello F, Simeone M, Sorrentino R, Rengo S, Spagnuolo G, Procino A
J Oral Sci. 2016 Dec 28

Read offline:

Related Articles:

	In vivo 2-color multiphoton imaging of genetic reporter…Fibroblasts are cells that synthesize collagen and support the maintenance of connective tissue in the skin, tendons, and joints. They maintain a critical role in the immune system’s response to…
	Why photobiomodulation therapy is helpful for diabetic wound…It is an obvious well-known issue that diabetic wounds are poorly healing, but the molecular and cellular alterations leading to this impairment is not clear. Many studies concluded the dysfunction…
	How can we tuned magnetic response of a composite particles…Composite particles contain two or more components and therefore they could have several advantages over single component particles, as multifuntionality, novel functions or enhanced properties. Their properties are induced by…
	Sustainable solutions: Tamarind Seed Kernel powder (TKP)…The majority of petroleum-based adhesives applied in the wood industry today, particularly formaldehyde-based adhesives. Their application trend is increasing due to their wide availability, superior bonding, durability, and resistance to…
	Soft-particle gel electrophoresisParticle electrophoresis in free solution or in gel media is a powerful technique for characterizing the surface properties of colloidal particles. Particle gel electrophoresis, that is, transport processes of colloidal…
	Modulation of intracellular dopamine signaling by…Aripiprazole, a dopamine partial agonist, is one of the most widely prescribed atypical antipsychotics and its use in children and adolescents has become common. However, due to its favorable side…