This study was aimed to assess the potential effects of ethanolicMentha piperita(MP) extract on the hemato – immunological and biochemical parameters, skin antibacterial activity and survival rate againstYersinia ruckeriinfection in rainbow troutOncorhynchus mykiss. Fish (32.2 ± 2.2 g) were divided into 4 groups before being fed diets supplemented with 0, 1, 2 and 3% of MP plant extract for 8 weeks., The RBC counts and haemoglobin concentrations showed a significant increase in 2 and 3% MP extracts enriched diet feeding group compared to the control and 1% MP extract enriched diet treated group.

Fig. 1. Mentha piperita.

同时,血细胞压积水平的迹象ificantly increased on fish fed diets enriched with 2 and 3% of MP extract and 3% group shown the highest Ht. Additionally, there were significant increases of WBC levels of fish fed with 3% MP extract when compared to the control and 1% MP extract groups. Thus, neutrophil percentage were also higher in fish feeding with 3% MP extract compared to the control and 1% MP extract groups. The results of the total protein showed that 3% MP extract group had a significant difference compared to the control and 1% groups. The albumin value was significantly enhanced in fish fed 2% and 3% MP extract when compared to the control and 1% groups. The lysozyme activity were significantly increased in all the groups of fish fed MP extract enriched diets, respect to the values found in control group, being the highest observed increments in those fish fed 2 and 3% enriched diets of MP extract. The serum total Ig values of 2 and 3% MP extract groups were significantly higher than the control and 1% groups and 3% group showed the highest serum total Ig value. The respiratory burst activity was significantly enhanced in all the groups of fish fed MP extract enriched diets when compared to the control group and the highest respiratory burst activity was found in 3% group. The serum total peroxidase content after 8 weeks showed that 2 and 3% MP extract groups had a significant difference compared to the control and 1% groups. Feeding on MP extract -supplemented diets caused a significant increase in antibacterial activity compared to the control group. The growth of all the bacteria species tested (S. iniae, Y. ruckeri, A. hydrophila and L. garviea) was significantly inhibited when mucus were obtained from fish fed MP extract -supplemented diet, compared to the values find from fish of control group. The inhibition was always higher in those mucus samples from fish fed the higher MP extract concentration (2 and3%). The results of the MIC of rainbow trout skin mucus were similar to those of antimicrobial activity. Furthermore, dietary MP extract supplements have no significant effect on alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, glucose, triglyceride, lymphocytes and monocytes levels of rainbow trout. After 8 weeks the cessation of feeding with MP extract, survival rates of 54.4%, 63.6% and 75.2% were recorded in groups which received 1, 2 and 3% of MP extract of feed, respectively, compared to 34.6% survivals in the control. This study underlying several positive effects of dietary administration of MP plant extract to farmed fish.

Milad Adel¹,Reza Pourgholam²,Jalil Zorriehzahra¹,Maryam Ghiasi^2
1Iranian Fisheries Research Organization,
Agriculture Research Education and Extension organization, Tehran, Iran
²Caspian Sea Ecology Research Center, Iranian Fisheries Research Organization,
Agriculture Research Education and Extension organization, Sari, Iran

Publication

Hemato – Immunological and biochemical parameters, skin antibacterial activity, and survival in rainbow trout (Oncorhynchus mykiss) following the diet supplemented with Mentha piperita against Yersinia ruckeri.
Adel M, Pourgholam R, Zorriehzahra J, Ghiasi M
Fish Shellfish Immunol. 2016 Aug

Read offline:

相关文章:

	Bioactive extracts from Posidonia oceanica waste biomass:…Bioactive compounds with antioxidant and antimicrobial activity have found applications in a broad range of areas within the pharmaceutical and food industries. As a result, the prospection of new biomass…
	A mixture of plant extracts attenuates postprandial glycemiaThe epidemiological studies demonstrate beneficial effects of a regular consumption of fruits, vegetables or grains on human health, which is usually a result of their lower caloric value and dietary…
	New antimicrobial strategies against multidrug-resistant…Due to current antibiotic resistance worldwide, there is an urgent need to find new alternative antibacterial approaches capable of dealing with multidrug-resistant pathogens. In fact, the World Health Organization has…
	The noxious effects of benthic diatoms on marine…Diatoms dominate various marine and freshwater environments, at any latitude, and are responsible for up to 25% of primary production. They may be either free-living in the water column or…
	Silkworms turning your shirt into a cell phone batteryFeeding Bombyx mori larvae with nanostructured materials such as CNTs, graphene, TiO2 and other metal oxides have been investigated in recent reports. The feeding process proved that Bombyx mori larvae…
	Essential fatty acids in the vegan/total vegetarian dietPeople choose vegetarian/vegan diets and for many reasons, however, these diets require careful dietary planning. The essential polyunsaturated n-6 (omega-6) and n-3 (omega-3) fatty acids are required for synthesis of…