Fluoride (F) is widely available in nature and has been successfully used to control dental caries worldwide. In addition, beneficial effects have been shown on the development of teeth and bones. However, high concentrations of F over time can cause side effects such as fluorosis that can affect both teeth and soft tissues due to disturbances in cellular processes and pathways, such as signaling pathways, mitochondrial energy metabolism and redox status. Since F alters energy metabolism, a common effect associated with overexposure to F is oxidative stress, which, if not contained, can lead to cell death. Thus, our study investigated the changes in proteins related to mitochondrial metabolism, after exposure to F in drinking water for 20 or 60 days, according to the groups (control – only water; 15 mgF/L or 50 mgF/L). For this, we performed proteomics analysis of rat liver mitochondria.

Graphical abstract

这项工作的主要结果表明,direction of the energy flow depends on the F dose and the exposure time. The dose of 15 mgF/L when administered for 20 days leads to a reduction in glycolysis, which is counterbalanced by the increase in β-oxidation, the urea cycle and the electron transport chain (ETC). For the same dose in the period of 60 days, there is an increase in all the energy pathways of the mitochondria. It is important to highlight that the excessive release of electrons during ETC is one of the causes of the generation of ROS, since 90% of the O₂consumed is used in oxidative phosphorylation (OXPHOS) to generate ATP. To compensate for the generation of reactive oxygen species (ROS) due to the vigorous state of energy production, there is a concomitant increase in antioxidant enzymes (SOD, PHB, GPx, PRX and CAT) and anti-apoptotic proteins such as ACAA2. On the other hand, the dose of 50 mgF/L when administered for 20 days reduced the proteins involved in all energy pathways. With less energy production, there is less ROS generation, leading to a reduction in antioxidant enzymes. However, when 50 mgF/L was administered for 60 days, an increase in energy metabolism was observed, though, antioxidant enzymes, in general, did not show changes in expression. Also, our findings related to Ca²⁺homeostasis and apoptosis that were altered after exposure to F generally showed an attempt to maintain Ca²⁺homeostasis and prevent mitochondrial apoptosis. The increase in Calmodulin protein (in treated groups/both periods-except for the highest dose of F for 20 days), contributes to the inactivation of mCU activity, preventing the accumulation of Ca²⁺in the mitochondria. On the other hand, regardless of the period, the dose of 15 mgF/L showed an increase in VDAC, which is responsible for the release of mitochondrial products, which can lead to apoptosis that was counterbalanced by the increase in ACAA2, responsible for inhibiting pro-apoptotic proteins. As a result, mitochondrial damage could result in an energy imbalance due to reduced mitochondrial respiration, excessive ROS generation and reduced ATP synthesis that can lead to cell death. However, the increase in ACAA2 in high energy flow groups appears to have helped to prevent greater mitochondrial damage.

Additional studies using longer periods with higher doses of F should be performed, so that the relationship between energy production and oxidative balance can be better clarified. Thus, our results, when analyzed together, suggest that the organism seems to adapt to the effects of F over time, activating ways to reduce its toxicity. Ultimately, our findings confirm the safety of using F for caries control.

Tamara T. Araujo, Marília A.R. Buzalaf
Bauru School of Dentistry, University of Sao Paulo, Brazil

Publication

Changes in energy metabolism induced by fluoride: Insights from inside the mitochondria
塔玛拉TeodoroAraujo, Heloisa Aparecida倒钩osa Silva Pereira, Aline Dionizio, Camila do Carmo Sanchez, Thamyris de Souza Carvalho, Mileni da Silva Fernandes, Marília Afonso Rabelo Buzalaf
Chemosphere. 2019 Dec

Read offline:

Related Articles:

	Modulation of intracellular dopamine signaling by…Aripiprazole, a dopamine partial agonist, is one of the most widely prescribed atypical antipsychotics and its use in children and adolescents has become common. However, due to its favorable side…
	Autophagy suppression decreases craniofacial bone massMacroautophagy (hereafter autophagy) is the main intracellular degradation mechanism to maintain cellular homeostasis by degrading misfolded proteins, recycling dysfunctional organelles, and generating energy fuels under physiological and pathological conditions. Autophagy…
	When will my mouse die?The maintenance of health is ensured by the function of the three homeostatic systems of the body: the nervous, endocrine and immune systems, and by the net that allows crosstalk…
	Chronic unpredictable environmental stress is harmful to…Stress refers to the mental and emotional change of state from normal homeostasis. In another words we can say that stress happens when we are worried for something and feeling…
	Soft-particle gel electrophoresisParticle electrophoresis in free solution or in gel media is a powerful technique for characterizing the surface properties of colloidal particles. Particle gel electrophoresis, that is, transport processes of colloidal…
	The SETOC: Visualizing carcinogenesis through a different…The Systemic-Evolutionary Theory of the Origin of Cancer (SETOC) is a recently proposed theory based on two fundamental principles: (i) Margulian evolution as a process of cooperation and endosymbiosis and…